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Abstract A limitation in fine-tuned tree-ring radiocarbon (14C) data is normally associated with overall data uncertainty. Tree-ring14C data variance as a result of sample heterogeneity can be reduced by adopting best practices at the time of sample collection and subsequent preparation and analysis. Variance-reduction of14C data was achieved by meticulous sample handling during increment core or cross-sectional cuttings, in-laboratory wood reductions, and cellulose fiber homogenization of whole rings. To demonstrate the performance of those procedures to final14C results, we took advantage of the replicated data from assigned calendar years of two Pantropical post-1950 AD tree-ring14C reconstructions. TwoCedrela fissilisVell. trees spaced 22.5 km apart, and two trees of this species together with onePeltogyne paniculataBenth tree spaced 0.2 to 5 km apart were sampled in a tropical dry and moist forest, respectively. Replicate14C data were then obtained from grouped tree-ring samples from each site. A total of 88% of the replicated14C results fell into a remarkably consistent precision/accuracy range of 0.3% or less, even though multiple tree species were used as pairs/sets. This finding illustrates how adopting a few simple strategies, in tandem with already established chemical extraction procedures and high-precision14C analysis, can improve14C data results of tropical trees.more » « lessFree, publicly-accessible full text available December 10, 2025
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